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Fluid dynamics characterisation of a rotating bioreactor for tissue engineering

Abstract : Biological scaffolds composed of extracellular matrix (ECM) derived from decellularised tissue are increasingly used in regenerative medicine. In this project, a flow perfusion bioreactor (the rotary cell culture system (RCCS), commercially available from Synthecon (Houston, TX)) is used in order to obtain some esophageal extracellular matrix. A theoretical mechanical characterisation of this experimental setup is provided. Due to the combination of rotation and perfusion, some spiral Poiseuille flow is created inside the tubular esophagus. In a transverse section, a particle (or cell) experiences simultaneously gravitational, Archimedes, centrifugal, Coriolis, and drag forces. In a frame of reference rotating with angular velocity , the particle follows a periodic nearly circular path in the clockwise direction, associated with a very slow centrifugal drift towards the esophagus wall. It appears that moderate perfusion rate and rotation speed ( < 20 rpm and Q < 30 ml/min) are appropriate experimental conditions for esophagus tissue engineering using the RCCS Synthecon bioreactor.
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Submitted on : Monday, June 20, 2022 - 4:14:13 PM
Last modification on : Monday, August 29, 2022 - 11:53:03 AM
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Agnès Drochon, Romane Lesieur, Marlène Durand. Fluid dynamics characterisation of a rotating bioreactor for tissue engineering. Medical Engineering & Physics, Elsevier, 2022, 105, pp.103831. ⟨10.1016/j.medengphy.2022.103831⟩. ⟨hal-03699926⟩



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