Multiscale-Engineered Muscle Constructs: PEG Hydrogel Micro-Patterning on an Electrospun PCL Mat Functionalized with Gold Nanoparticles - Archive ouverte HAL Access content directly
Journal Articles International Journal of Molecular Sciences Year : 2022

Multiscale-Engineered Muscle Constructs: PEG Hydrogel Micro-Patterning on an Electrospun PCL Mat Functionalized with Gold Nanoparticles

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Megane Beldjilali-Labro
  • Function : Author
Rachid Jellali
Alexander David Brown
  • Function : Author
Alejandro Garcia Garcia
  • Function : Author
Augustin Lerebours
  • Function : Author
  • PersonId : 1128298
Erwann Guénin
Fahmi Bedoui
Murielle Dufresne
  • Function : Author
  • PersonId : 1117039
Claire Stewart
  • Function : Author
  • PersonId : 1128299
Jean-François Grosset
  • Function : Author
  • PersonId : 1128300
Alexander Brown
  • Function : Author
C. Legallais

Abstract

The development of new, viable, and functional engineered tissue is a complex and challenging task. Skeletal muscle constructs have specific requirements as cells are sensitive to the stiffness, geometry of the materials, and biological micro-environment. The aim of this study was thus to design and characterize a multi-scale scaffold and to evaluate it regarding the differentiation process of C2C12 skeletal myoblasts. The significance of the work lies in the microfabrication of lines of polyethylene glycol, on poly(ε-caprolactone) nanofiber sheets obtained using the electrospinning process, coated or not with gold nanoparticles to act as a potential substrate for electrical stimulation. The differentiation of C2C12 cells was studied over a period of seven days and quantified through both expression of specific genes, and analysis of the myotubes’ alignment and length using confocal microscopy. We demonstrated that our multiscale bio-construct presented tunable mechanical properties and supported the different stages skeletal muscle, as well as improving the parallel orientation of the myotubes with a variation of less than 15°. These scaffolds showed the ability of sustained myogenic differentiation by enhancing the organization of reconstructed skeletal muscle. Moreover, they may be suitable for applications in mechanical and electrical stimulation to mimic the muscle’s physiological functions.
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Dates and versions

hal-03590377 , version 1 (27-02-2022)

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Megane Beldjilali-Labro, Rachid Jellali, Alexander David Brown, Alejandro Garcia Garcia, Augustin Lerebours, et al.. Multiscale-Engineered Muscle Constructs: PEG Hydrogel Micro-Patterning on an Electrospun PCL Mat Functionalized with Gold Nanoparticles. International Journal of Molecular Sciences, 2022, 23 (1), pp.260. ⟨10.3390/ijms23010260⟩. ⟨hal-03590377⟩
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