Additive manufacturing process creates local surface roughness modifications leading to variation in cell adhesion on multifaceted TiAl6V4 samples - Université de technologie de Compiègne Access content directly
Journal Articles International Journal of Bioprinting Year : 2019

Additive manufacturing process creates local surface roughness modifications leading to variation in cell adhesion on multifaceted TiAl6V4 samples

Abstract

This work looks at the effect of the surface texture variability on cell adhesion and cell morphology of an osteoblastic linage (MC3T3) using a multifaceted TiAl6V4 (stem-like) specimen produced by selective laser melting. We produced a Ti6Al4V anchoring component presenting de facto surfaces with different inclinations with respect to the build plate. Surfaces had different complex surface textures composed of submicroscale groove tracks induced by the laser beam, and partially melted particles varying with the inclination of the surface in the SLM chamber. We measured the adhesion of cells on the specimen, having previously tested any cytotoxic effect of the material on a fibroblastic linage. Our multifaceted geometric specimens made it possible to compare potential effects of different surface textures on the same specimen. We found that cell attachment increased on surfaces with microscale grooves, and that partially melted powders were the least appreciated zones for adhesion (26-fold increase in the number of cells from a zone containing 1192 particles/mm2 compared to 188,3 particles/mm2). The ratio of the number of submicroscale grooves to the number of partially melted particles can be controlled by the inclination of the surface with respect to the build plate. Surfaces with a lower inclination (upskin surfaces) had fewer partially melted particles than downskin surfaces (200 particles/mm2 at 35° compared to 1200 particles/mm2 at 125°). Based on our results, we propose an approach to better understand and therefore control the inherent heterogeneities of AM-based implants in order to reduce potentially costly post-processing for osseous interface implant.
Fichier principal
Vignette du fichier
S2405886618300472.pdf (310.42 Ko) Télécharger le fichier
Origin Files produced by the author(s)

Dates and versions

hal-02407732 , version 1 (21-12-2021)

Licence

Identifiers

Cite

Augustin Lerebours, Pascale Vigneron, Salima Bouvier, Alain Rassineux, Maxence Bigerelle, et al.. Additive manufacturing process creates local surface roughness modifications leading to variation in cell adhesion on multifaceted TiAl6V4 samples. International Journal of Bioprinting, 2019, 16, pp.e00054. ⟨10.1016/j.bprint.2019.e00054⟩. ⟨hal-02407732⟩
131 View
123 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More