Skip to Main content Skip to Navigation
Journal articles

In Vitro Bone Cell Response to Tensile Mechanical Solicitations: Is There an Optimal Protocol?

Abstract : Bone remodeling is strongly linked to external mechanical signals. Such stimuli are widely used in vitro for bone tissue engineering by applying mechanical solicitations to cell cultures so as to trigger specific cell responses. However, the literature highlights considerable variability in devices and protocols. Here the major biological, mechanical, and technical parameters implemented for in vitro tensile loading applications are reviewed. The objective is to identify which values are used most, and whether there is an optimal protocol to obtain a functional tissue-engineering construct. First, a shift that occurred from fundamental comprehension of bone formation, to its application in rebuilt tissues and clinical fields is shown. Despite the lack of standardized protocols, consensual conditions relevant for in vitro bone development, in particular cell differentiation, could be highlighted. Culture processes are guided by physiological considerations, although out-of-range conditions are sometimes used without implying negative results for the development of rebuilt tissue. Consensus can be found on several parameters, such as strain frequency (1 Hz) or the use of rest periods, but other points have not yet been fully established, especially synergies with other solicitations. It is believed that the present work will be useful to develop new tissue-engineering processes based on stretching.
Complete list of metadata
Contributor : Cecile Legallais Connect in order to contact the contributor
Submitted on : Friday, December 18, 2020 - 3:50:30 PM
Last modification on : Tuesday, March 15, 2022 - 3:20:03 AM
Long-term archiving on: : Friday, March 19, 2021 - 6:11:02 PM


Revised MS with highlights 081...
Files produced by the author(s)




Timothée Baudequin, Cécile Legallais, Fahmi Bedoui. In Vitro Bone Cell Response to Tensile Mechanical Solicitations: Is There an Optimal Protocol?. Biotechnology Journal, Wiley-VCH Verlag, 2019, 14 (1), pp.1800358. ⟨10.1002/biot.201800358⟩. ⟨hal-02061207⟩



Record views


Files downloads