Glue embolization is a minimally invasive treatment used to block the blood flow to specific targeted sites. Cyanoacrylate liquid glues, mixed with radiopaque iodized oil, have been widely used for vascular embolization owing to their low viscosity, rapid polymerization rate, good penetration ability and low tissue toxicity. In this study, we have conducted an in vitro study to quantitatively investigate the polymerization kinetics of two n-butyl cyanoacrylate (nBCA) glues (Glubran 2 and Histoacryl) mixed with an iodized oil (Lipiodol) at various concentrations. The polymerization process of the glue-oil mixture is systematically characterized upon contact with a protein ionic solution mimicking plasma and compared to the case without protein. The results provide essential information for interventional radiologists to help them understand the glue behavior upon injection, and thus control embolization.