Sequential and simultaneous strategies for biorefining of wheat straw using room temperature ionic liquids, xylanases and cellulases

Eric Husson 1 Thomas Auxenfans 2 Mickaël Herbaut 2 Manon Baralle 2 Virginie Lambertyn 1 Harivoni Rakotoarivonina 2 Caroline Remond 2 Catherine Sarazin 1
1 GEC - Génie Enzymatique et Cellulaire
2 FARE - Fractionnement des AgroRessources et Environnement - UMR-A 614
Abstract : Sequential and simultaneous strategies for fractioning wheat straw were developed in combining 1-ethyl-3-methyl imidazolium acetate [C2mim][OAc], endo-xylanases from Thermobacillus xylanilyticus and commercial cellulases. After [C2mim][OAc]-pretreatment, hydrolysis catalyzed by endo-xylanases of wheat straw led to efficient xylose production with very competitive yield (97.6 ± 1.3%). Subsequent enzymatic saccharification allowed achieving a total degradation of cellulosic fraction (> 99%). These high performances revealed an interesting complementarity of [C2mim][OAc]-and xylanase-pretreatments for increasing enzymatic digest-ibility of cellulosic fraction in agreement with the structural and morphological changes of wheat straw induced by each of these pretreatment steps. In addition a higher tolerance of endo-xylanases from T. xylaniliticus to [C2mim][AcO] until 30% v/v than cellulases from T. reesei was observed. Based on this property, a simultaneous strategy combining [C2mim][OAc]-and endo-xylanases as pretreatment in a one-batch produced xylose with similar yield than those obtained by the sequential strategy.
Keywords : Ionic liquid Sequential and simultaneous process Lignocellulosic biomass Cellulase Xylanase
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Submitted on : Wednesday, January 23, 2019 - 2:16:01 PM
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Eric Husson, Thomas Auxenfans, Mickaël Herbaut, Manon Baralle, Virginie Lambertyn, et al.. Sequential and simultaneous strategies for biorefining of wheat straw using room temperature ionic liquids, xylanases and cellulases. Bioresource Technology, Elsevier, 2018, 251, pp.280-287. ⟨10.1016/j.biortech.2017.12.047⟩. ⟨hal-01988880⟩

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