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Université de Technologie de Compiègne |
Journal articles
Sequential and simultaneous strategies for biorefining of wheat straw using room temperature ionic liquids, xylanases and cellulases
Abstract : Sequential and simultaneous strategies for fractioning wheat straw were developed in combining 1-ethyl-3-methyl imidazolium acetate [C2mim][OAc], endo-xylanases from Thermobacillus xylanilyticus and commercial cellulases. After [C2mim][OAc]-pretreatment, hydrolysis catalyzed by endo-xylanases of wheat straw led to efficient xylose production with very competitive yield (97.6 ± 1.3%). Subsequent enzymatic saccharification allowed achieving a total degradation of cellulosic fraction (> 99%). These high performances revealed an interesting complementarity of [C2mim][OAc]-and xylanase-pretreatments for increasing enzymatic digest-ibility of cellulosic fraction in agreement with the structural and morphological changes of wheat straw induced by each of these pretreatment steps. In addition a higher tolerance of endo-xylanases from T. xylaniliticus to [C2mim][AcO] until 30% v/v than cellulases from T. reesei was observed. Based on this property, a simultaneous strategy combining [C2mim][OAc]-and endo-xylanases as pretreatment in a one-batch produced xylose with similar yield than those obtained by the sequential strategy.
Cited literature [52 references]
https://hal.utc.fr/hal-01988880
Contributor : Stephane Octave <>
Submitted on : Wednesday, January 23, 2019 - 2:16:01 PM
Last modification on : Friday, December 18, 2020 - 3:46:02 PM
Contributor : Stephane Octave <>
Submitted on : Wednesday, January 23, 2019 - 2:16:01 PM
Last modification on : Friday, December 18, 2020 - 3:46:02 PM