Analysis of 13 C labeling amino acids by capillary electrophoresis – High resolution mass spectrometry in developing flaxseed

Sébastien Acket 1 Anthony Degournay 1 Mathilde Gosset 1 Franck Merlier 1 Manual Adrian Troncoso-Ponce 1 Brigitte Thomasset 1
1 GEC - Génie Enzymatique et Cellulaire
Abstract : Accurate assessment of labeling measurements Amino acids Metabolic flux analysis Fluxomic Flaxseed A B S T R A C T In context of fluxomic studies, 13 C labeling analysis of amino acids are very important for solving the carbon flow calculation, because they are synthesized in various biosynthesis pathways and cellular compartments in plant cells. Traditionally, 13 C labeling analysis are performed using low resolution mass spectrometry detector by GC-MS. We compared a method using capillary electrophoresis-high resolution mass spectrometry without deri-vatization and with better accuracy assessment of labeling measurements comparing to classical GC-MS. Our method allowed us to show that valine, leucine, alanine are not synthesized from the same pyruvate pool during the period of reserves accumulation in flax seeds.
Keywords : Fluxomic 13C labeling Flaxseed Accurate assessment of labeling measurements Metabolic flux analysis Amino acids
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Submitted on : Wednesday, January 23, 2019 - 2:14:32 PM
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Sébastien Acket, Anthony Degournay, Mathilde Gosset, Franck Merlier, Manual Adrian Troncoso-Ponce, et al.. Analysis of 13 C labeling amino acids by capillary electrophoresis – High resolution mass spectrometry in developing flaxseed. Analytical Biochemistry, Elsevier Masson, 2018, 547, pp.14-18. ⟨10.1016/j.ab.2018.02.009⟩. ⟨hal-01988869⟩

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