Competitive fluorescent pseudo-immunoassay exploiting molecularly imprinted polymers for the detection of biogenic amines in fish matrix

Abstract : We developed a competitive fluorescent molecularly imprinted polymer (MIP) assay to detect biogenic amines in fish samples. MIPs synthesized by precipitation polymerization using histamine as template were used in a batch binding assay analogous to competitive fluoroimmunoassays. Introducing a complex sample matrix, such as fish extract, into the assay changes the environment and the binding conditions, therefore the importance of the sample preparation is extensively discussed. Several extraction and purification methods for fish were comprehensively studied, and an optimal clean-up procedure for fish samples using liquid-liquid extraction was developed. The feasibility of the competitive MIP assay was shown in the purified fish extract over a broad histamine range (1 − 430 µM). The MIP had the highest affinity towards histamine, but recognized also the structurally similar biogenic amines tyramine and tryptamine, as well as spermine and spermidine, providing simultaneous analysis and assessment of the total amount of biogenic amines.
Document type :
Journal articles
Complete list of metadatas

Cited literature [52 references]  Display  Hide  Download

https://hal.utc.fr/hal-01988852
Contributor : Stephane Octave <>
Submitted on : Wednesday, January 23, 2019 - 2:06:00 PM
Last modification on : Friday, June 21, 2019 - 7:56:04 AM

Identifiers

Collections

Citation

Leena Mattsson, Jingjing Xu, Claudia Preininger, Bernadette Tse Sum Bui, Karsten Haupt. Competitive fluorescent pseudo-immunoassay exploiting molecularly imprinted polymers for the detection of biogenic amines in fish matrix. Talanta, Elsevier, 2018, 181, pp.190-196. ⟨10.1016/j.talanta.2018.01.010⟩. ⟨hal-01988852⟩

Share

Metrics

Record views

23