Untargeted Analysis of Semipolar Compounds by LC-MS and Targeted Analysis of Fatty Acids by GC-MS/GC-FID: From Plant Cultivation to Extract Preparation - Archive ouverte HAL Access content directly
Book Sections Year : 2018

Untargeted Analysis of Semipolar Compounds by LC-MS and Targeted Analysis of Fatty Acids by GC-MS/GC-FID: From Plant Cultivation to Extract Preparation

(1) , (2) , (2) , , (3) , (1) , (1)
1
2
3
Camille Benard
  • Function : Author
  • PersonId : 1204576
Sébastien Acket
  • Function : Author
  • PersonId : 1099963
  • IdRef : 185457843
Yannick Rossez
Olivier Fernandez
  • Function : Author
Yves Gibon
Cécile Cabasson

Abstract

The way plants are grown and samples are harvested, prepared, and extracted has a profound impact on the output of a metabolomics experiment. In this chapter, we detail the experimental procedures from plant cultivation to extract preparation, in order to avoid difficulties that could result in contamination or undesired changes of the analytes. Two plant organs are mentioned as examples: tomato fruits (Solanum lycopersicum) and flax seeds (Linum usitatissimum). Extractions designed for the untargeted analysis of semipolar compounds by liquid chromatography-mass spectrometry (LC-MS) and targeted analysis of fatty acids by gas chromatography-mass spectrometry (GC-MS) or gas chromatography with flame ionization detector (GC-FID) are described.
Not file

Dates and versions

hal-01972166 , version 1 (07-01-2019)

Identifiers

Cite

Camille Benard, Sébastien Acket, Yannick Rossez, Olivier Fernandez, Thierry Berton, et al.. Untargeted Analysis of Semipolar Compounds by LC-MS and Targeted Analysis of Fatty Acids by GC-MS/GC-FID: From Plant Cultivation to Extract Preparation. Plant Metabolomics, 1778, Springer Science + Business Media B.V., pp.101-124, 2018, Methods in Molecular Biology, 978-1-4939-7818-2. ⟨10.1007/978-1-4939-7819-9_8⟩. ⟨hal-01972166⟩
54 View
0 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More